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Fig. S2. Establishment of the temporal hierarchy in the activation of Gata5 targets. (A-C) A hormone-inducible version of Gata5 (Gata5-GR) was injected into the animal pole of two-cell stage embryos and the animal caps were dissected at blastula stage. Half of the Gata5-injected and uninjected explants were exposed to dexamethasone for 2 hours at three developmental stages (stage 11/gastrula; stage 18/neurula; stage 30/tailbud) before being processed for RT-PCR analysis. ODC was used as loading control, Sox17α and Pdx1 as positive controls for Gata5 induction at gastrula and tailbud stages, respectively. Image published in: Spagnoli FM and Brivanlou AH (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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