XB-IMG-149526
Xenbase Image ID: 149526
|
|
Fig. S3. Morpholinos targeting two distinct xTGIF2 pseudoalleles. (A) Alignment of Xenopus tropicalis TGIF2 and the two Xenopus laevis TGIF2 sequences targeted by the antisense morpholino oligonucleotides used in this study (yellow). The ATG is indicated in bold. Morpholino antisense oligonucleotides were directed against the 5′UTR of the two Xenopus laevis TGIF2 pseudoalleles: MO-1 (5′-CAGGGGGAGATTCAAGGAAGATGAA-3′) and MO-2 (5′-CCTAGTGATAGACCACGAGATGGAC-3′). Standard control morpholino corresponds to 5′-CCTCTTACCTCAGTTACAATTTATA-3′. In green is the Xenopus tropicalis TGIF2 morpholino sequence that was used in a recent systematic MO-based functional screen carried out in Xenopus tropicalis. In the X. tropicalis morphants, general phenotypic defects, including shortening of the A-P axis and defects in anterior and dorsal tissues, were described (Rana et al., 2006). Similar defects were also visible in TGIF2-Mo-injected embryos (see Fig. 4B). (B) Xenopus TGIF2 translation from the mRNA encoding xTGIF2 allele 1 is specifically inhibited by TGIF2-MO1, but not by control MO. 10 µM morpholino was added to the in vitro translation reactions.
Rana, A. A., Collart, C., Gilchrist, M. J. and Smith, J. C. (2006). Defining synphenotype groups in Xenopus tropicalis by use of antisense morpholino oligonucleotides. PLoS Genet. 2, e193. Image published in: Spagnoli FM and Brivanlou AH (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |