Xenbase Image ID: 149555

Fig. 4. Dishevelled activates Daam1, leading to gastrulation defects, and activated Daam1 rescues convergent extension defects. (A) Expression of subthreshold doses of Dsh (100 pg of RNA) or Daam1 (1 ng of RNA) does not interfere with gastrulation, but coinjection of these subthreshold doses of Dsh and Daam1 induces gastrulation defects. Embryos were injected into both dorsal blastomeres, and embryos were scored at stage 35. Embryos with an open blastopore, exposed endodermal tissue, and significantly reduced anterior–posterior (AP) axis were scored as severe embryos (Middle Left), and embryos with a small open blastopore or delayed blastopore closure and a slightly shortened AP axis or bent body axis were scored as mild. (B) Quantitation of the phenotypes of injected embryos in A. (C) Gastrulation in Xenopus embryos is inhibited by expression of dominant negative Dsh (Xdd1) (1 ng of RNA), dominant negative XWnt11 (DN-Wnt11) (2 ng of RNA), or Daam1 MO (100 ng), but this inhibition is rescued by C-Daam (100 pg of DNA) or DaamδDAD (100 pg of DNA) coinjection but not Daam1 (1 ng of RNA) coinjection. (D) Quantitation of the results of phenotypic analysis in C. In B and D, the number of embryos scored (n) is shown at the top of each bar. Image published in: Liu W et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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