Xenbase Image ID: 149564

Fig. 7. Rho activation is required for stress fiber induction by C-Daam1. Daam1ΔDAD can induce Rac activation. (A and B) Quantitation of the results from Fig. 3A. (C) Rho activity is required for stress fiber induction by C-Daam1. Transfected NIH 3T3 cells were transfected with GFP-Daam1, GFP-C-Daam1, or GFP-Daam1DDAD and incubated in the presence of a cell-permeable Rho inhibitor, and stress fiber induction was monitored by phalloidin staining. Note that the Rho inhibitor did not suppress cell protrusions induced by GFP-DaamDDAD. (D) Quantitation of the results of C. (E) Rac assay shows that Dvl2 and DaamDDAD but nor Daam1 or C-Daam1 can induce Rac activation in HEK293T cells. Image published in: Liu W et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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