Xenbase Image ID: 149674

Fig. 6. The extracellular domain of E-cadherin is required for CNC migration in vivo. (A) Embryos were injected with indicated constructs and in situ hybridization with AP2 was performed at stage 25. (B) E-cadherin constructs used in the rescue experiments. S: signal peptide; EC: extracellular cadherin (repeat); TM: transmembrane domain; p120 and β-cat: p120 catenin and β-catenin binding site. (C) Statistic of the in situ hybridization. N=number of experiments and n=number of embryos. * Indicates injected site and bars show average percentage of embryos with CNC migration defect. At least three independent experiments were performed and the average percentage is shown with standard deviations. Significance is calculated according to fisher’s exact test. Scale bar: 200 μm. Image published in: Huang C et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tfap2a.L ap2, AP2a, AP2alpha, tfap2a-a, tfap2a-b, tfap2alpha, XAP-2, xap2 X. laevis Throughout NF stage 27 neural crest cranial neural crest mandibular crest hyoid crest branchial arch head head mesenchyme Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + cdh1 MO + NF25 (in situ hybridization) fig.6. a, c Anatomical Phenotype abnormal cell migration in neural crest Larger Image Printer Friendly View

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