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Fig. S4. Specificity of the E-cadherin antisense morpholino oligonucleotide. (A) Endogenous E-cadherin expression was significantly reduced in E-cadMO injected embryos, but not in wild type embryos or embryos injected with control morpholino (CoMO). (B) Rescue construct of E-cadherin (E-cadMu) is expressed in the embryo and does not bind the E-cadherin morpholino. 4 Embryos were injected with mRNA of unmutated full length E-cadherin with a HA-tag (E-cadfl-HA) alone or along with E-cadMO. Expression of the MO binding site mutated E-cadherin rescue construct (E-cadMu-HA) is not affected by E-cadMO co-injection. Expression of Cadherin-11 (C), N-cadherin (D) and XB-cadherin (E) are not affected in E-cadherin morphant embryos. Immunodetection was performed on cell lysates against the HA-tag, myc-tag, XB-cadherin and α-Tubulin. WT: uninjected wild type embryos. Anti-α-Tubulin was served as loading control. Image published in: Huang C et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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