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Fig. S6. Analysis of apoptosis in E-cadherin knockdown embryos by TUNEL assay. Embryos were injected with either 8 ng CoMO or 8 ng E-cadMO at 16-cell stage and fixed at stage 17 or stage 23, followed by whole mount TUNEL assay. Apoptosis was not significantly increased in E-cadMO morphant embryos compared to CoMO injected embryos at (A) stage 17 or (B) stage 23. (C) Statistics of the whole mount TUNEL assay. * Indicates the injected site. Significance is calculated according to fisher’s exact test. Scale bar: 200 μm. Image published in: Huang C et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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