Xenbase Image ID: 149694

Fig. 3. Sf3b4MO, a translation blocking morpholino prevents snail2, sox10 and twist expression. (A) Design of the translation blocking morpholino antisense oligonucleotide. Sf3b4MO (red bar) targets the ATG start site of Xenopus Sf3b4 mRNA. (B) Western blot using lysates from embryos injected with XSf3b4 mRNA alone or in combination with Sf3b4MO, shows that Sf3b4MO blocks SF3B4 protein accumulation in vivo. α-tubulin is shown as a loading control. (C)Snail2 expression is reduced in Sf3b4MO-injected (40 ng) embryos (arrows). Dorsal views, anterior to top. The injected side (arrows) is indicated by the lineage tracer (Red-Gal). (D) The graph is a quantification of the results from three independent experiments. The number of embryos analyzed (n) is indicated on the top of the bar. (E) Gene expression analysis (qPCR) of control (U) and Sf3b4MO-injected embryos. Values are normalized to ef1α and presented as mean ± s.e.m.; (*) p < 0.002 (Student’s t-test), from five independent samples. Image published in: Devotta A et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 15 cranial neural crest Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + sf3b4 MO + NF15 (in situ hybridization) fig.3.c Expression Phenotype decreased amount snai2.L expression in cranial neural crest Larger Image Printer Friendly View

Return to previous page