XB-IMG-149715
Xenbase Image ID: 149715
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Fig. S3. Phagocytes enwrap myelin dystrophies and increase lipid droplets during metamorphosis. (A) Phagocyte processes contact and enwrap focal myelin
protrusions and detachments on remodeling metamorphic axons. Subsets of axon reconstructions from SBEM datasets for stages 62 and 66 shown in Fig. 2D
and Fig. S2D (boxed regions) show myelin (light and dark blue), axoplasm (yellow), focal periaxonal space enlargements (white), and phagocyte processes (red).
(Scale bar: 5 μm.) (B) Maximum projections of confocal-imaged ON longitudinal cryosections immunolabeled with Mbp (blue) and large-caliber axon marker
3A10 (white), with discrete Mbp structures not associated with axons (pseudocolored red). (Scale bar: 50 μm.) (C) Percentage of Mbp not colocalizing with 3A10
per ON (n = 10) based on 3D reconstructions of confocal datasets. *P < 0.05; ***P < 0.001 (by the Games–Howell test). (D) Area of lipid droplets relative to total
myelin area per ON cross-section, measured from traced TEM micrographs in n = 6 ON per stage, showing mean ± SD. *P < 0.05 by the Games–Howell test. (E)
TEM micrograph showing degrading myelin and lipid droplet accumulation within an astrocyte in stage 66 ON. (Scale bar: 2 μm.) Image published in: Mills EA et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |