XB-IMG-149716
Xenbase Image ID: 149716
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Fig. S4. A-F. Astrocytes express phagocytic genes in the metamorphic ON. (A) IB4-lectin-FITC–labeled microglia (green), laminin-immunolabeled ON sheath (red),
and DAPI-labeled nuclei labeled (blue) in ON longitudinal cryosections, showing that IB4-lectin+ microglia infiltrate the ON parenchyma in stage 60 animals 3 d
after ON transection. (Scale bar: 50 μm.) (B) IB4-lectin-FITC signal per unit area in the ON core 3 d after ON transection, per ON (n = 5). *P < 0.05 by Tukey’s test.
(C) Immunofluorescence due to Tg(Blbp:Cd63-mCherry) transgene (red) in ON cross-sections at stages 58 and 66 shows this lysosomal marker accumulating in
processes of Blbp-immunolabeled astrocytes (green). (Scale bar: 50 μm.) (D) mCherry signal for Tg(Blbp:Cd63-mCherry) per unit area is elevated at stage 66.
Measures show mean ± SD (n = 10 ON per stage). *P < 0.05 by the Games–Howell test. (E) Immunofluoresence due to Tg(Blbp:Mettl7b-mCherry) transgene
(red), a lipid droplet marker, accumulates in processes of Blbp-immunolabeled astrocytes (green) during metamorphic climax. (Scale bar: 50 μm.) (F) mCherry
signal, derived from the Tg(Blbp:Mettl7b-mCherry) transgene, per unit area is elevated during metamorphic climax. Measurement shows mean ± SD (n = 10 ON
per stage). *P < 0.05 by Tukey’s test. Image published in: Mills EA et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |