Xenbase Image ID: 149719

Fig. S5. TRΔC blocks TH-mediated gene expression in a cell type-specific manner. (A) T3-induced gene 12 mRNA (red) up-regulation by T3 in the ON is blunted in the ON of Tg(Blbp:rtTA/TetOp:GFP-TRΔC) animals. In Tg(Mbp:rtTA/TetOp:GFP-TRΔC) animals, total gene 12 expression is not inhibited, because mature oligodendroctyes, identified by expression of plp (green), only weakly express gene 12. (Scale bar: 50 μm.) (B) T3-induced gene 12 mRNA (red) is primarily in astrocytes, identified by immunolabeling for Blbp (green). (Scale bar: 50 μm.) (C) Gene 12 mRNA signal mean fluorescence intensity in all cells of ON (n = 6, n = 8, or n = 5 ON in WT, Blbp, or Mbp, respectively). *P < 0.05; **P < 0.01 (by the Games–Howell test). (D) Total myelin per ON cross-section at stage 66 (n = 16 for controls and n = 8 ONs for TRΔC). **P < 0.01 by Dunnett’s test. Image published in: Mills EA et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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