Xenbase Image ID: 149834
Figure 2. Reverse transcription-polymerase chain reaction (RT-PCR) reveals characteristic temporal expression patterns of six 14-3-3 family members in normal developing embryos. Total RNA was purified from normal developing embryos between stage 2 and 38 and used in RT-PCR experiments with established primers for specific 14-3-3 isoforms. The 14-3-3 β, τ, and ϵ were the most abundantly expressed isoforms throughout early Xenopus embryonic development. Three other 14-3-3 genes, η, γ, and ζ, were expressed at much lower levels in embryos. The 14-3-3-β gene expression level was relatively constant between stages 2 and 38. The 14-3-3 ϵ and τ shared similar gene expression patterns: mRNA levels slowly decreased during blastula and gastrula stages (stage 2 through 11.5) but increased during neurula stages (after stage 14). The 14-3-3 η and ζ mRNA levels were both low between stages 2 and 17 and expression was up-regulated only in later stages. The 14-3-3 γ had a variable mRNA expression pattern, which peaked around stages 20 and 31. ornithine decarboxylase (ODC) expression was analyzed as an equal loading control. Data are representative of two experiments with two separate sets of specific primers for each 14-3-3 family member.
Image published in: Lau JM et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons.
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