XB-IMG-149836
Xenbase Image ID: 149836
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Figure 5. Injection of 14-3-3 family member-specific morpholinos produced unique phenotypic abnormalities in Xenopus embryos. A: Two-cell embryos were injected with a total of 10 pmol of family member-specific morpholinos into both blastomeres of two-cell embryos. a: Embryos injected with nonspecific control morpholino developed normally through tadpole stages; b: 14-3-3 β morpholino-injected embryos showed slight ventralization defects but were otherwise normal; c: 14-3-3 ϵ-specific morpholino injection resulted in frequent exogastrulation defects; d: 14-3-3 γ-specific morpholino injection resulted in a small eye or no eye phenotype; e,f: 14-3-3 η- or ζ-specific morpholino injection did not affect embryogenesis. B: Failure of blastopore lip closure and subsequent exogastrulation in embryos injected with 14-3-3 τ morpholinos. The embryos were traced from the beginning of gastrulation at stage 10.5 (a), through the end of gastrulation at stage 12 (b), to stage 17 at the end of neurulation (c). d,e: Normally developing embryos at stage 32–33 (d) and stage 39–40 (e). f,g: Embryos injected unilaterally with 10 pmol of 14-3-3 τ morpholinos at stage 32–33 (f) and stage 39–40 (g). h,i: Embryos injected diagonally with 10 pmol of 14-3-3 τ morpholinos at stage 32–33 (h) and stage 39–40 (i) Image published in: Lau JM et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |