Xenbase Image ID: 149838

Figure 7. Microinjection of 14-3-3 τ mRNA rescues animal cap elongation and exogastrulation defects in 14-3-3 τ morpholino-injected embryos. Two-cell stage embryos were either uninjected, injected with 5 pmol of 14-3-3 τ-specific morpholino, or first injected with 5 pmol of 14-3-3 τ-specific morpholino, followed by injection of 50 ng of myc-14-3-3 τ mRNA or myc-14-3-3 η mRNA. A: Embryos injected with 14-3-3 τ morpholino had inhibited cap elongation that could be partially rescued (69%) by co-injection of 14-3-3 τ mRNA. Co-injection using a different 14-3-3 mRNA construct (14-3-3 η) was less effective (36%) in rescuing the cap elongation defects caused by 14-3-3 τ morpholino treatment. Results are the mean ± SEM from three separate experiments. B: Embryos co-injected with 14-3-3 τ or ϵ mRNA had reduced rate of exogastrulation (75 ± 4.5% and 84 ± 15.9%, respectively) compared with those injected with 14-3-3 τ morpholino alone (100%, P < 0.05 for both as determined by Student's t-test). Results were combined from four separate experiments. Image published in: Lau JM et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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