Xenbase Image ID: 149839

Figure 8. Developmental defects in 14-3-3 τ-specific morpholino-injected embryos were associated with increased apoptosis. A: Embryos injected with 14-3-3 τ- or ϵ-specific morpholinos displayed much higher rate of apoptosis than control embryos at the corresponding stage. Two-cell stage embryos were either uninjected (orange line), injected with 14-3-3 τ- specific morpholino (purple), or injected with 14-3-3 ϵ-specific morpholino (blue). Apoptosis was scored using an enzyme-linked immuosorbent assay (ELISA) apoptosis kit (Roche) and quantified by measuring double absorbance at 415/490 nm. Results are representative of two separate experiments. B: Co-injection of 14-3-3 τ mRNA with 14-3-3 τ morpholinos reduced the apoptotic rate in Xenopus embryos. Lysates were harvested from stage 12 embryos that were uninjected, 14-3-3 τ mRNA-injected, 14-3-3 τ morpholino-injected, or morpholino / mRNA co-injected. Apoptosis was increased in 14-3-3 τ morpholino-injected embryos; co-injection of 14-3-3 τ mRNA or 14-3-3 ζ mRNA reduced the rate of apoptosis. Injection of 14-3-3 τ mRNA alone did not affect apoptosis. Results were pooled from five separate experiments. Image published in: Lau JM et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

Return to previous page