Xenbase Image ID: 149975

Figure 1: Properties of Noggin4. (a) Alignment of Xenopus laevis Noggins. Positions essential for Noggin1 binding to BMP1 are indicated by red arrows. HSPG binding motifs and cleavage sites are indicated by green and red dashed lines, respectively. (b) Whole-mount in situ hybridization of a midneurula embryo with dig-probe to Noggin4 mRNA. Dorsal view at the top, the right-frontal view at the bottom. (c) Effects of Noggin4 mRNA (80%, n = 120) and MO1 (85%, n = 130) injections on the development of head structures. Cg-cement gland. (d–s) In situ hybridization with dig-probes to FoxG1, Rx, Otx2 and Pax6 mRNA of midneurula embryos injected with GFP mRNA (control) (0%, n = 40; 0%, n = 50; 0%, n = 40; 0%, n = 30, abnormal phenotypes respectively), Noggin4 mRNA (85%, n = 30; 61%, n = 35; 81%, n = 40; 65%, n = 40 abnormal phenotypes respectively), Noggin4 MO1 (90%, n = 50; 86%, n = 40; 60%, n = 40; 60%, n = 40, abnormal phenotypes respectively) and misNoggin4 MO1 (control) (0%, n = 30; 0%, n = 30; 0%, n = 40; 0%, n = 30, abnormal phenotypes respectively). Red dashed line surrounds the presumptive rostral forebrain territory. Anterior view, dorsal to the top. Image published in: Eroshkin FM et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue foxg1.L bf1, foxg1b, LOC108700624, XBF-1, XBf1 X. laevis Throughout NF stage 15 pre-chordal neural plate anterior neural ridge pre-chordal neural plate border rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 15 eye primordium pre-chordal neural plate otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 15 eye primordium pre-chordal neural plate pre-chordal neural plate border pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 15 eye primordium pre-chordal neural plate chordal neural plate nog4.S nog-4, noggin 4 X. laevis Throughout NF stage 15 pre-chordal neural plate anterior neural fold pre-chordal neural plate border Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + nog + NF32 (morphology) fig.1.c Anatomical Phenotype abnormal development of head abnormal head morphology increased size of the cement gland increased size of the eye Xla Wt + nog4 MO + NF32 (morphology) fig.1.c Anatomical Phenotype abnormal development of head decreased size of the cement gland decreased size of the eye Xla Wt + nog + NF15 (in situ hybridization) fig.1.e Expression Phenotype increased amount foxg1.L expression in pre-chordal neural plate Xla Wt + nog4 MO + NF15 (in situ hybridization) fig.1.f Expression Phenotype decreased amount foxg1.L expression in pre-chordal neural plate Xla Wt + nog + NF15 (in situ hybridization) fig.1.i Expression Phenotype increased amount rax.L expression in pre-chordal neural plate Xla Wt + nog4 MO + NF15 (in situ hybridization) fig.1.j Expression Phenotype decreased amount rax.L expression in pre-chordal neural plate Xla Wt + nog + NF15 (in situ hybridization) fig.1.m Expression Phenotype decreased amount otx2.S expression in pre-chordal neural plate increased amount otx2.S expression in pre-chordal neural plate border Xla Wt + nog4 MO + NF15 (in situ hybridization) fig.1.n Expression Phenotype decreased amount otx2.S expression in pre-chordal neural plate Xla Wt + nog + NF15 (in situ hybridization) fig.1.q Expression Phenotype increased amount pax6.L expression in pre-chordal neural plate Xla Wt + nog4 MO + NF15 (in situ hybridization) fig.1.r Expression Phenotype decreased amount pax6.L expression in pre-chordal neural plate Larger Image Printer Friendly View

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