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Figure 6: Investigation of Noggin4 interaction with Wnt8 in living embryos via FRAP assay. (a) The cartoon explains how free diffusion of Noggin4 is retarded by its interaction with Wnt8 adsorbed on HSPG. (b) Schema for obtaining embryos that express EGFP-Noggin4 and TagRFP-Wnt8 in different cells. (c) Representative examples of FRAP in the intracellular space of animal ectoderm in embryos injected, as shown in B, with mRNAs of indicated fluorescently labelled proteins. Arrows mark the borders of the bleached region. (d) Mean values of the effective diffusion coefficients of EGFP-Noggin4 and EGFP-hep measured experimentally and shown in (a–c). Five to 7 series of experiments (10 to 20 individual measurements per experiment) were conducted for each of the four types of injections. Averaged FRAP curves are presented at Fig. S15. Image published in: Eroshkin FM et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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