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Figure S3. Effects of Noggin4 mRNA and MO2 injections on tadpole head structures and expression of anterior markers, FoxG1 and Rx, at the midneurula stage. (a–c) 5 days tadpoles injected as indicated. (d–f) Heads of 5 days tadpoles injected as indicated are shown from the dorsal side, anterior to the top. (g-l’) In situ hybridisation with dig-probes to FoxG1 and Rx mRNA of midneurula embryos injected with control MO provided by GeneTools (100%, n=30 (g and g’)), Noggin4 mRNA (85%, n=30 (h and h’) and 61%, n=35 (k and k’)), misNoggin4 MO2 (100%, n=40 (i and i’, l and l’)), and misNoggin4 MO1 (100%, n=40 (f) and 80%, n=40 (i)). Image published in: Eroshkin FM et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue foxg1.L bf1, foxg1b, LOC108700624, XBF-1, XBf1 X. laevis Throughout NF stage 14 pre-chordal neural plate pre-chordal neural plate border anterior neural ridge rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 14 optic field pre-chordal neural plate Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + nog4 + NF47 (morphology) fig.S3.b, e Anatomical Phenotype abnormal eye morphology increased pigmentation in the head increased pigmentation in the optic stalk increased size of the barbel Xla Wt + nog4 MO + NF47 (morphology) fig.S3.c Anatomical Phenotype abnormally laterally curved whole organism Xla Wt + nog4 MO + NF47 (morphology) fig.S3.c, f Anatomical Phenotype abnormal eye morphology abnormal head morphology abnormally medially mislocalised eye absent barbel Xla Wt + nog4 + NF15 (in situ hybridization) fig.S3.h Expression Phenotype increased amount foxg1.L expression in anterior neural ridge Xla Wt + nog4 MO + NF15 (in situ hybridization) fig.S3.i Expression Phenotype decreased amount foxg1.L expression in anterior neural ridge Xla Wt + nog4 + NF15 (in situ hybridization) fig.S3.k Expression Phenotype increased amount rax.L expression in pre-chordal neural plate Xla Wt + nog4 MO + NF15 (in situ hybridization) fig.S3.l Expression Phenotype decreased amount rax.L expression in pre-chordal neural plate Larger Image Printer Friendly View

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