Xenbase Image ID: 149985

Figure S4. Testing of MO specificity and efficiency. (a) Schema of the morpholino target sites location on both pseudoalleles of the Xenopus laevis Noggin4 mRNA. (b) 5’-UTR-Myc-Noggin4 mRNA were injected into each blastomere of 2-cell Xenopus laevis embryos (100 pg/blastomere), either alone or in a mixture with control misNoggin4 MO, MO1, MO2 (8 nl of 0.2 mM water solution) or the standard control MO provided by GeneTools (8 nl of 0.5 mM water solution). The injected embryos were collected at the midgastrula stage and analysed for presence of Myc-Noggin4 by Western blotting with anti-Myc antibody (see Materials and Methods for details). (c-d’) The specificity of the MO effects was confirmed by rescue experiments, in which Noggin4 MO1 was injected either alone (c and c’) or with Noggin4 mRNA deprived of the MO1 target site (d and d’). Medial Dorso-Anterior Index (DAI) was calculated for both types of tadpoles developed from the injected embryos. Image published in: Eroshkin FM et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Experiment + Assay Source Phenotypes and Disease Xla Wt + nog4 MO + NF47 (morphology) fig.S4.c Anatomical Phenotype decreased length of anterior-posterior axis decreased size of the tail edematous ventral trunk increased size of the head wholly anteriorized embryo Larger Image Printer Friendly View

Return to previous page