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Figure S5. Noggin4 acts cell-non-autonomously when promoting the expression of anterior markers. (a) Control embryo injected in dorsal blastomeres with EGFP mRNA mixed with FLD tracer at 8-cell stage and hybridised at the midneurula stage with FoxG1 probe. This embryo is presented for comparison of width of the control FoxG1 expression band with width of FoxG1 expression band in the embryo injected with Noggin4 mRNA and shown on b. (b-f) Midneurula embryos injected with Noggin4 mRNA mixed with FLD tracer unilaterally, in one of the animal dorsal blastomeres, at 8-cell stage and hybridised with probes to the indicated marker genes. Arrows indicate areas in which overexpression of the tested marker genes does not match the localisation of FLD, thereby confirming cell-non-autonomous activity of Noggin4. (a’-f’) Fluorescent images of the same embryos as on a–f. (a”–f”) Superimposed images of a–f and a’–f’. (g–g”). An expanded cement gland on the non-injected side of the tail-bud stage embryo (right embryo), which was injected in the right animal dorsal and ventral blastomeres at 8-cell stage, confirms cell-non-autonomous activity of Noggin4. Image published in: Eroshkin FM et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue foxg1.L bf1, foxg1b, LOC108700624, XBF-1, XBf1 X. laevis Throughout NF stage 14 anterior neural ridge pre-chordal neural plate pre-chordal neural plate border rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 14 optic field pre-chordal neural plate otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 14 optic field pre-chordal neural plate pre-chordal neural plate border pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 14 optic field pre-chordal neural plate chordal neural plate Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + nog4 + NF15 (in situ hybridization) fig.S5.b Expression Phenotype increased amount foxg1.L expression in anterior neural ridge Xla Wt + nog4 + NF15 (in situ hybridization) fig.S5.d Expression Phenotype increased amount rax.L expression in pre-chordal neural plate Xla Wt + nog4 + NF15 (in situ hybridization) fig.S5.e Expression Phenotype increased amount otx2.S expression in pre-chordal neural plate Xla Wt + nog4 + NF15 (in situ hybridization) fig.S5.f Expression Phenotype increased amount pax6.L expression in pre-chordal neural plate Xla Wt + nog4 + tailbud stage (morphology) fig.S5.f Anatomical Phenotype increased size of the cement gland Larger Image Printer Friendly View

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