Xenbase Image ID: 149989

Figure S8. Fusions of Noggin1, Noggin2 and Noggin4 with EGFP retain their molecular and physiological activities. (a) EGFP-Noggin1 and EGFP-Noggin2 inhibit expression of the BMP-specific luciferase reporter, BRE, in Xenopus laevis embryos. The embryos were injected with BRE reporter plasmid, either alone (control) or mixed with EGFP-Noggin1 or EGFP-Noggin2 mRNAs. (b) EGFP-Noggin4 inhibits expression of the Wnt/b-Catenin-specific luciferase reporter, TOPFlash, in Xenopus laevis embryos. The embryos were injected either with TOPFlash reporter mixed with XWnt8 mRNA (control) or with TOPFlash reporter plasmid mixed with XWnt8 mRNA and increasing concentrations of EGFP-Noggin4 mRNA. (c and d). EGFP-Noggin4 mRNA co-injected with mRNA of the BMP inhibitor, truncated BMP receptor tBR, induces development of secondary trunk and head structures (better visible on c), including cyclopic eyes, in Xenopus laevis tadpoles (50%, n=70). Two different sets of tadpoles (obtained in the independent experiments) were photographed at stage 39 and 46 respectively. On c two upper tadpoles are shown from the right side and the bottom tadpole is shown from the ventral side. On d two upper tadpoles are shown from the right side and the bottom tadpole from the left side. Image published in: Eroshkin FM et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Experiment + Assay Source Phenotypes and Disease Xla Wt + nog4-EGFP + dnbmpr1a + NF39-46 (morphology) fig.S8.c,d Anatomical Phenotype abnormally increased number of eye decreased length of tail duplicated head duplicated trunk obsolete duplicated anterior-posterior axis Larger Image Printer Friendly View

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