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Figure S16. Relative concentrations of the endogenous Noggin4 and EGFP-Noggin4 mRNAs in the ectodermal explants of the midgastrula stage embryos. 70 pg of EGFP-Noggin4 mRNA was injected into the animal pole cytoplasm of each dorsal blastomere of 4-cell stage embryos. The dorsal ectoderm explants were excised and collected from injected and wild-type, non-injected, sibling embryos at stage 11, followed by RNA extraction. qRT-PCR with primers for Noggin4 and EGFP was performed for samples obtained from non-injected and injected embryos, respectively. The data were normalised relative to expression of two housekeeping genes, ODC and EF1a as described in.5 The expression level of the endogenous Noggin4 in the wild-type samples was used for normalisation and regarded as one unit. Image published in: Eroshkin FM et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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