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XB-IMG-150100

Xenbase Image ID: 150100


Fig. 3. BMPpathway overactivation affects cell specification in the developing Xenopus epidermis. Stage 9 Xenopus embryos were injected in the blastocoel with either BSA (A-M) or 2-7 ng recombinant BMP4 (A′-M′). (A,A′) SEM of the epidermis at stage 37 revealed the severe decrease in the numbers of MCCs (red arrowhead), ionocytes (yellow arrowhead) and SSCs (green arrowhead). (B,B′) At tailbud (stage 30), BMP4-injected embryos showed normal morphology but substantially fewer α-tubulin-positive MCCs, as revealed by whole-mount in situ hybridisation (WISH). (C,C′) Both the differentiated MCC marker acetylated tubulin (white) and the ionocytemarker foxi1e (green)were lost in sectioned tailbud (stage 25)BMP4-injected embryos. (D,D′)WISHrevealed that the differentiated ionocyte marker v1awas strongly decreased in stage 25BMP4-injectedembryos. (E-F′) The differentiatedSSCsmarkers serotonin (red in E,E′) andtryptophan hydroxylase-1 (tph1, red in F,F′)were lost or strongly decreased in stage 25BMP4-injected embryos. (G,G′) In sections of stage 25BMP4-injected embryos, P63 (white), amarker of the non-intercalatinginner layercells,was lost,whereasthe goblet cellmarker intelectin-1 (green)wasupregulated. otogelin (redin H,H′,J,J′)andtrim29(red in I,I′), two other markers of goblet cells, were unaffected. (K-M′) Stage 14 embryos were analysed by WISH in order to stain MCCs for foxj1 (K,K′), ionocytes for foxi1e (L,L′) and SSCs for foxa1 (M,M′). (B,B′,D-F′,J-M′) Whole-mount embryos; (C,C′,G-I′) cryosectioned embryos. (B-M′) All markers were revealed by chromogenic or fluorescent in situ hybridisation, except for acetylated tubulin, serotonin and P63, which were revealed by immunofluorescence. (C,C′,E-I′) Nuclei were stained with DAPI. (B,B′,D-F′,K-M′) The number of embryos showing the phenotype among the total number of embryos examined is indicated.

Image published in: Cibois M et al. (2015)

Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneCloneSynonymsSpeciesStage(s)Tissue
tuba4b.L tuba4, alpha tubulin, alpha-tubulin, tuba4aX.laevisThroughout NF stage 29 and 30multiciliated cell
epidermis
ciliated epidermal cell
tuba4b.L tuba4, alpha tubulin, alpha-tubulin, tuba4aX.laevisThroughout NF stage 25epidermis
multiciliated cell
ciliated epidermal cell
foxi1.L Xema, ectodermally-expressed mesendoderm antagon, FoxI1e, xfoxi1, ema, foxi1e, foxi3X.laevisThroughout NF stage 25epidermis
ionocyte
tph1.L X.laevisThroughout NF stage 25secretory epithelial cell
tp63.L trp63, ket, tp73l, Xp63, p63, np63X.laevisThroughout NF stage 25epidermis
epidermis inner layer
itln1.L lfr, hl-1, intl, itln, omentin, xeel, hl1X.laevisThroughout NF stage 25secretory epithelial cell
mucus secreting cell
epithelium
otogl2.L otogelin, otgn, mlemp, XEP-1X.laevisThroughout NF stage 25secretory epithelial cell
mucus secreting cell
epithelium
trim29.L X.laevisThroughout NF stage 25mucus secreting cell
secretory epithelial cell
epithelium
foxj1.L xfoxj1, xfoxj1, hfh4, hfh-4, foxj1-b, foxj1-aX.laevisThroughout NF stage 14multiciliated cell
epidermis
foxi1.L Xema, ectodermally-expressed mesendoderm antagon, FoxI1e, xfoxi1, ema, foxi1e, foxi3X.laevisThroughout NF stage 14ionocyte
epidermis
foxa1.L xfkh2, HNF3alpha, HNF-3 alpha, HNF3 alpha, HNF3-alpha, tFoxA1, hnf3a, tcf3a, fkh2, foxa1-a, foxa1-b, foxa1bX.laevisThroughout NF stage 14secretory epithelial cell
epidermis
atp6v1a.L ho68, va68, vpp2, vma1, atp6a1, atp6v1a1, v1a, atpv1aX.laevisThroughout NF stage 25epidermis
ionocyte

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