Xenbase Image ID: 150156

Fig. S1. Immunodetection of endogenous Vangl2 protein. (A, B) Basolateral localization (arrowhead) of Vangl2 (A) and b1-integrin (B) in embryonic ectoderm at stage 11. (C) Immunoblot of ectodermal cell lysates with anti- Vangl2 antibody reveals a 60 kDa band (arrowhead) corresponding to endogenous Vangl2, misexpressed Xenopus CFP-Vangl2 and mouse HA-VL2, as indicated.(D-G) Vangl2 immunostaining on cross-sections of st. 10- and st. 10.5 embryos. Dashed boxes in D and E correspond to the magnified images shown in D’ and E’. Vangl2 is detected at the basolateral membrane and in cytoplasmic vesicles near the apical cortex, and appears more abundant in the superficial cell layer. Scale bar in A is 20 μm. Both apical (arrow) and basolateral staining is detected in the constricting blastopore cells of st. 10.5 embryo. D-G, Scale bar is 20 μm. Image published in: Ossipova O et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue vangl2.L lpp1, ltap, stb1, stbm, stbm1, strabismus, Van Gogh, van gogh-like2, vang, vang-like 2, vangl2-a, vangl2-b, Xstrabismus X. laevis Throughout NF stage 11 ectoderm itgb1.L Beta 1 integrin, cd29, fnrb, gpiia, itgb1-a, itgb1-b, mdf2, msk12, vla-beta, vlab X. laevis Throughout NF stage 11 ectoderm vangl2.L lpp1, ltap, stb1, stbm, stbm1, strabismus, Van Gogh, van gogh-like2, vang, vang-like 2, vangl2-a, vangl2-b, Xstrabismus X. laevis Throughout NF stage 10.5 blastopore bottle cell vangl2.L lpp1, ltap, stb1, stbm, stbm1, strabismus, Van Gogh, van gogh-like2, vang, vang-like 2, vangl2-a, vangl2-b, Xstrabismus X. laevis Throughout NF stage 10 epithelial cell involuting marginal zone epithelial layer animal cap outer layer Image source: Published Larger Image Printer Friendly View

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