Xenbase Image ID: 150160

Fig. S5. Vangl2 is dissociated from the cell membrane in Rab11-depleted embryos. Embryos were coinjected with CO MO or Vangl2 MO (30 ng each) and 100 pg of GFP RNA as lineage tracer (not shown). A, B, Morphology of control (A) and Rab11-depleted (B) embryos at st. 11, vegetal views are shown. C, D, Rab11 depletion interferes with the membrane localization of Vangl2, including the apical surface of the constricting blastopore cells (arrow). E, F, Basolateral membrane localization of Vangl2 is inhibited in ectoderm cells depleted of Rab11. Scale bar in E, also refers to C, D and F, is 20 μm. Image published in: Ossipova O et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue vangl2.L lpp1, ltap, stb1, stbm, stbm1, strabismus, Van Gogh, van gogh-like2, vang, vang-like 2, vangl2-a, vangl2-b, Xstrabismus X. laevis Throughout NF stage 11 blastopore lip bottle cell vangl2.L lpp1, ltap, stb1, stbm, stbm1, strabismus, Van Gogh, van gogh-like2, vang, vang-like 2, vangl2-a, vangl2-b, Xstrabismus X. laevis Throughout NF stage 11 ectoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + rab11a MO + NF11 (morphology) fig.s5.b Anatomical Phenotype abnormal development of blastopore Xla Wt + rab11a MO + NF11 (morphology) fig.s5.d Expression Phenotype mislocalised vangl1.L expression in bottle cell Xla Wt + rab11a MO + NF11 (morphology) fig.s5.f Expression Phenotype mislocalised vangl1.L expression in ectodermal cell decreased amount vangl1.L expression in ectodermal cell Larger Image Printer Friendly View

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