XB-IMG-150234
Xenbase Image ID: 150234
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Figure 2. MiR-449 affects actin network remodelling and multiciliogenesis in Xenopus epidermis.(a–d) The epidermis precursor blastomeres (eight-cell stage Xenopus embryos) were injected with negative control morpholinos (CTR-Neg) (a,c), with morpholinos against miR-449 (449-MOs) (a,b) or with protector morpholinos of Dll1 (PO-Dll1) (c,d). Staining at stage 25 for F-Actin (in red, a1,5 and c1,5) and motile cilia (Ac. Tub. in magenta, a3,7 and c3,7). Injected cells were detected by the expression of a synthetic mRNA coding for membrane-bound GFP (GFP-CAAX in green, a2,6 and c2,6). (b) The histogram indicates the percentage of GFP-CAAX-positive injected cells that develop motile cilia or apical actin cap in controls (Stage 24+25: n=5 fields/583 injected cells) and in miR-449 morphants (Stage 24+25: n=8 fields/625 injected cells; P-value st.24+25=0.0087; Mann–Whitney test with two-tailed P-value). (d) Percentage of injected cells positive for GFP fluorescence with normal or defective actin cap in control (n=30 fields per 1,345 injected cells) versus PO-Dll1 morphants (n=32 fields per 1,268 injected cells; P-value (normal versus defective in control) st.24+25 <0.0001, P value (normal versus defective in PO-Dll1-injected st.24+25=0.0033; Mann–Whitney test with two-tailed P-value). (e) Effect of protecting the Dll1 mRNA from the interaction with miR-449 (PO-Dll1) in Xenopus epidermis on Dll1 expression (normalized with ornithine decarboxylase (ODC)). (f) Effect of PO-Dll1 in Xenopus epidermis on miR-449 expression, normalized with U6. Data are means±s.d. of two independent experiments. Image published in: Chevalier B et al. (2015) Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |