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FIGURE 4:. Comparative in situ hybridization analysis confirms species-specific localization in X. laevis and X. tropicalis oocytes. (A–C) In situ hybridization with species-specific antisense RNA probes was performed with stage I–IV oocytes from X. laevis and X. tropicalis. (A) Gdf1, grip2, gplt, and cnksr2 localize to the vegetal cortex in both X. laevis and X. tropicalis oocytes. (B) Ppp1r2, pgam1, atrx, and tob2 vegetally localize in X. laevis only. (C) Mogat1, pld2, acp6, and krt8 transcripts localize to the vegetal cortex in X. tropicalis but not X. laevis oocytes. Image published in: Claußen M et al. (2015) © 2015 Claußen et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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