Xenbase Image ID: 150404

Figure 2. XFoxJ1 morpholinos inhibits ciliogenesis in Xenopus skin cells.(A-D) Xenopus embryos injected with both XFoxJ1 morpholinos, or a control morpholino were stained at stage 26 with ZO-1 (red) and anti-acetylated tubulin (green) to label cell borders and cilia, respectively (A,B), or were injected with RNAs encoding a membrane-localized RFP (red) and a GFP-centrin2 fusion protein (green), to label cell membranes and basal bodies, respectively (C,D). (E) Control or FoxJ1 morphants as above were co-injected with RNA encoding a GFP-centrin2 fusion protein (green), fixed at stage 26 and stained with rhodamine-phallodin (red) to label the apical actin network. Bottom panels show a 2μm Z-scan through the apical domain. Scale bars represent 20μm in A-B and 10μm in C-E. Image published in: Stubbs JL et al. (2008) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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