Xenbase Image ID: 150406

Figure 4. Bi-ciliate cells on the GRP and induced ectopically by XFoxJ1(A-B) Shown is a confocal image of the skin at stage 26 of embryos injected with RFP RNA (A) or with both FoxJ1 and RFP RNA (B), and stained with an antibody to γ-tubulin (green). Arrows denote centriole number and position. (C-D) Confocal image of the uninjected GRP at stage 17, either (C) stained with antibodies to ZO-1 (Red) and acetylated-tubulin (green) or (D) with antibodies to ZO-1 (Red) and γ-tubulin (green). Arrows indicate cilia number (C) or centriole position (D). Scale bars = 10μm. (E-F) Quantification of cilia number (E) and split centrioles (F) in the uninjected GRP at stage 17 (St.17 GRP, n=100 cilia or centriole pairs from each of 4 embryos), or in the outer epithelial cells (OCs) of stage 26 embryos that were injected with FoxJ1 RNA (St26/OCs/FoxJ1) or with just RFP RNA as a control (St26/OCs). For stage 26 n=200 cilia or centriole pairs from each of 4 embryos. Error bars represent S.D. Image published in: Stubbs JL et al. (2008) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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