XB-IMG-150407
Xenbase Image ID: 150407
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Figure 5. Validation of gene expression regulated by FoxJ1.(A-C) Shown is the roof of the gastrocoel in stage 17 embryos after staining for the expression of Tektin-t (A,A′), PF16 (B,B′) and L-R dynein (C,C′) RNA using whole-mount, in situ hybridization. Expression (Red-Blue stain) in the posterior GRP is marked with an arrow. Top panels show staining in uninjected embryos while lower panels shows that in embryos injected twice in one blastomere at the two-cell stage with XFoxJ1 RNA. Injected side is oriented to the right. (D) Embryos were injected at the two-cell stage with the indicated RNAs or with XFoxJ1 or control morpholinos. At stage 10, the ectoderm was isolated, cultured on fibronectin-coated glass to stage 22, and then extracted for total RNA. The levels of Tektin-t, PF16 (Spag6), or L-R dynein RNA was measured in each sample using quantitative PCR, and normalized relative to a ubiquitously expressed control RNA, ODC. Values for each experimental condition is an average of three measurements, and are expressed on a logarithmic plot as a ratio to the average value obtained with a control. Uninjected controls were used for the RNA injected samples and a control morpholino sample was used as a control for FoxJ1 morpholino injection. Image published in: Stubbs JL et al. (2008) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder.
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