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Fig 4. Modulation of Kv1.2 FRAKT by general anesthetics.(A) Sequence alignment of the S4-S5 linker from K-Shaw2 (314–332) and Kv1.2 (313–331) channels. Starting and ending residue numbers of the shown segments are indicated. In Kv1.2, the blue colored residues were swapped for the red colored residues in K-Shaw2 to create the Kv1.2 FRAKT mutant channel. (B) Effects of general anesthetics on whole-oocyte Kv1.2-FRAKT currents evoked by a voltage step to +60 mV from a holding voltage of -100 mV. Black, red and grey current traces correspond to control, anesthetic-exposed, and washout, respectively. The scale bars indicate 50 ms and 0.5 μA. (C) Concentration-response relations of various general anesthetics acting on the Kv1.2 FRAKT channel. Solid lines are the best fits assuming the Hill equation (propofol and n-butanol) or a double Hill equation (sevoflurane, isoflurane, and halothane) (Materials and Methods). Due to the small magnitude of the chloroform results, no reliable Hill equation fit could be obtained. N = 5–8 oocytes for each concentration. Best-fit parameters are summarized in Table 1. Image published in: Liang Q et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |