XB-IMG-150462
Xenbase Image ID: 150462
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Figure 3. Sleep is increased in Ca-α1T mutants.(a) Ca-α1T, Ca-α1TGal4, and Ca-α1TRescue schematics. Ca-α1T coding exons are red. Downward arrows denote the extent of the deleted region. SA, splice acceptor. pA, polyA sequence. (b) Western blot analysis of Ca-α1T protein levels of fly head lysates. Ca-α1T is undetectable in Ca-α1TGal4 lysates while Ca-α1TRescue lysates show levels similar to the w1118 control. β-actin was used as a loading control. (c) Sleep profiles of w1118 (black, n = 89), Ca-α1TGal4 (red, n = 92) and Ca-α1TRescue (grey, n = 61) over two days of 12 h:12 h light-dark (LD) and two days of continuous dark (DD) conditions. Sleep is plotted in 30 minute intervals. Data are presented as means ± s.e.m. White, black, and grey bars denote light phase, dark phase, and subjective light phase, respectively. ZT, zeitgeber time. CT, circadian time. (d) Total daily sleep under LD and DD conditions. (e) Waking activity under LD and DD conditions measured as total activity counts divided by waking minutes. (f) The number of sleep bouts under LD and DD conditions. (g) Average sleep bout length under LD and DD conditions. Boxplot whiskers extend to the highest and lowest values that fall within 1.5× IQR of the upper and lower quartiles. All indications of statistical significance were determined using Welch’s ANOVA followed by the Games-Howell post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001. Image published in: Jeong K et al. (2015) Copyright © 2015, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |