XB-IMG-150834
Xenbase Image ID: 150834
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Figure S2: Intron 1 of sall4 binds β-catenin but does not mediate a Wnt signal. (A) Using animal caps to screen for direct transcriptional targets of Wnt in neural tissue. (B) qPCR on 15-25 animal caps treated as indicated on the X-axis. The Y-axis shows expression relative to odc. meis3 and hoxb9 serve as controls for known direct and indirect targets of Wnt, respectively. (C) Quantification of dorsalization in uninjected embryos (open bars) and embryos injected animally with 500 pg FLAG- tagged β-catenin RNA (250 pg/blastomere) at the 2-cell stage (filled bars) as scored by the dorsoanterior index (DAI). Error bars: 1 SEM. Images show a representative uninjected (UC) embryo with a DAI of 7 (normal) and a representative embryo with a DAI of 6 (kinked axis). (D) Schematic of the genomic locus of sall4 in Xenopus laevis (Xenbase.org). Blue boxes indicate exons and yellow circles indicate the location of putative TCF/LEF binding sites. Black ovals show the locations of the zinc-finger domains. Numbers indicate the position of putative binding sites relative to the transcription start site (TSS). (E) Chromatin immunoprecipitation of FLAG-tagged β-catenin in late gastrulae/early neurulae. Open bars represent uninjected embryos and closed bars represent embryos injected with 500 pg FLAG- tagged β-catenin (250 pg/blastomere at the 2-cell stage). Error bars: 1 SEM per cent input for each measurement. (F) Luciferase reporter assays in HEK293 cells treated with or without BIO and/or mouse FGF. Error bars: 1 SEM. All means were compared by one-way ANOVA followed by Tukey post-hoc analyses (*: p<0.05).
Image published in: Young JJ et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |