Xenbase Image ID: 150836

Figure S4: sall1 is activated by canonical Wnt signaling and expressed during early embryogenesis. (A) qPCR on 5 whole embryos or 15 to 25 animal caps treated according to the conditions indicated on the X-axis. The Y-axis shows expression relative to odc. Error bars: 1 SEM. (B-E) Whole-mount in situ hybridizations of sall1 in Xenopus laevis embryos. (B) Whole mount stage 10 embryo stained for sall1, dorso-vegetal view with the dorsal lip of the blastopore oriented towards the top. (B’) Sagittal section of stage 10.5 embryo stained for sall1 expression, animal pole is to the top and dorsal is to the right. (C-D) Dorsal views of indicated neurula stage embryos, anterior is oriented towards the top. (C’-C’’) Transverse sections of stage 12 embryos stained for sall1, (C’) anterior and (C’’) posterior. (D’-D’’) Transverse sections of stage 15 embryos stained for sall1, (D’) anterior and (D’’) posterior. (E’-E’’) Transverse sections of stage 18 embryos stained for sall1, (E’) anterior and (E’’) posterior. (B’, C’-E’’) 50 μM sections, (C’-E’’) dorsal oriented towards the top. No: notochord, S: somite, PSM: presomitic mesoderm. Image published in: Young JJ et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sall1.L LOC108715370, sal-1, sal-like 1, sal1, sall1-a, sall1-b, sall1b, spalt-like 1, tbs, znf794 X. laevis Throughout NF stage 10 dorsal marginal zone ectoderm mesoderm sall1.L LOC108715370, sal-1, sal-like 1, sal1, sall1-a, sall1-b, sall1b, spalt-like 1, tbs, znf794 X. laevis Throughout NF stage 10.5 dorsal marginal zone upper blastopore lip involuted dorsal mesoderm sall1.L LOC108715370, sal-1, sal-like 1, sal1, sall1-a, sall1-b, sall1b, spalt-like 1, tbs, znf794 X. laevis Throughout NF stage 12 circumblastoporal collar mesoderm presumptive axial mesoderm sall1.L LOC108715370, sal-1, sal-like 1, sal1, sall1-a, sall1-b, sall1b, spalt-like 1, tbs, znf794 X. laevis Throughout NF stage 15 circumblastoporal collar mesoderm axial mesoderm posterior sall1.L LOC108715370, sal-1, sal-like 1, sal1, sall1-a, sall1-b, sall1b, spalt-like 1, tbs, znf794 X. laevis Throughout NF stage 18 posterior mesoderm notochord Image source: Published Larger Image Printer Friendly View

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