XB-IMG-150860
Xenbase Image ID: 150860
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Figure S3. (A) Schematic of fezf2 MO design and knockdown efficiency. The MO
targets the exon3-intron3 junction (above) and semi-quantitative PCR analysis shows
mature fezf2 mRNA levels in stage 24 embryos (below), injected with control MO (1)
or fezf2 MO (2). Black arrow pair indicates the position of validation primers for RTPCR
analysis, the forward primer overlaps the exon3-exon4 junction. (B) qPCR
results, n=3 replicates. In both cases, the ribosomal protein L8 (rpl8) gene was used
as an internal control. Image published in: Zhang S et al. (2014) © 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |