XB-IMG-150865
Xenbase Image ID: 150865
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Figure S8 (related to Figure 6). Fezf2 represses the activities of lhx2 and lhx9. (A-B)
Control experiments showing the expression of lhx2 and lhx9 in stage 12 animal cap explants injected with p3hGR-VP16-Fezf2, either left untreated, or treated with either
CHX or DEX alone. qPCR results of (A) lhx2 and (B) lhx9 are shown (n=3 replicates,
P<0.001). rpl8 was used as an internal control. (C-D) Stage 20 animal cap explants
neuralised by chordin mRNA expressed high level of lhx2 and lhx9. qPCR results of
(C) lhx2 and (D) lhx9 are shown (n=3 replicates, P<0.001 and P<0.05). rpl8 was used
as an internal control. (E) Bar graph of the quantification and statistics of phenotypes
from control or fezf2 morphant groups regarding the expression of lhx2 and lhx9. (F-G)
Validation of lhx2 i2e3 MO. (F) Validation by RT-PCR. M: marker. Lane 1-2: rpl8; 1:
control MO; 2: lhx2 MO. Lane 3-4: lhx2; Lane 3: control MO, Lane 4: lhx2 MO. (G)
Validation by qPCR. rpl8 was used as an internal control in both cases. n=3 replicates,
P<0.001. (H-I) Validation of lhx9 e1i1 MO. (H) Validation by RT-PCR. M: marker.
Lane 1-2: rpl8; 1: control MO; 2: lhx9 MO. Lane 3-4: lhx9; Lane 3: control MO, Lane
4: lhx9 MO. (I) Validation by qPCR. rpl8 was used as an internal control in both cases.
n=3 replicates, P<0.01. (J) Bar graph of the quantification and statistics of phenotypes
from different morphant groups regarding arx expression. (K) Bar graph of the
quantification and statistics of phenotypes from different morphant groups regarding
ngn1 expression. In all bar graphs, numbers above each bar represent counts of
embryos examined in each group. Error bars represent ±s. e. m. Image published in: Zhang S et al. (2014) © 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |