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Fig. 4 Off-target effects of CRISPR/Cas9 in X. tropicalis evaluated via T7E1 assay. a Representative gel image of T7E1 digested off-target amplicons. The percentage under panels indicated the frequencies of off-target effects. b Quantification of the cleavage frequencies of all off-target sites. No clear and specific bands after T7E1 assay were generated at sox9-579 and lrig3-E306 loci. The cutting efficiencies at sox9-579 and lrig3-E306 sites were calculated by sequencing results. Red dash line indicated 10 % in indels efficiency Image published in: Liu Z et al. (2016) © Liu et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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