Xenbase Image ID: 150927

Figure 4. Hermes/Rbpms interacts with another Nanos1 binding protein: To identify proteins that might interact with Hermes/Rbpms during early oogenesis, a Xenopus cDNA library (Clontech) was screened using a yeast two-hybrid (Y2H) system. (A) Full length Hermes/Rbpms fused with the DNA binding domain (BD) of the transcription factor Gal4 served as bait. Candidate interacting proteins were fused to the Gal4-activation domain (AD) and served as prey. The reporter gene B-galactosidase, driven by the Gal4 binding site, was positively transcribed as the result of Hermes/Rbpms and hnRNP I (heterogeneous nuclear ribonucleoprotein I) interaction. Two proteins involved in yeast glucose metabolism, Sfn4 and Sfn1, were used as controls to discard false positive results. Snf4/Gal4-BD was used as a bait and Snf1/Gal4-AD as a prey to check false interactions with hnRNP I and Hermes/Rbpms protein respectively; (B) The C-terminal 34 amino acids (in red) required for Hermes/Rbpms to dimerize and bind nanos RNA are not required to interact with hnRNP I. Hermes/Rbpms protein lacking the terminal 34 AA was used as bait and hnRNP I as prey. Induction of B-galactosidase indicated a positive interaction between bait and prey. Image published in: Aguero T et al. (2016) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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