Xenbase Image ID: 151022

S2 Fig. Rescue of pkdcc1Mo and pkdcc2Mo phenotype by pkdcc1(mut) and pkdcc2(mut) mRNAs, respectively. (A-E) Four cell stage embryos were injected dorsally with coMo (A), pkdcc1Mo (B) or pkdcc2Mo (D) and incubated until blastopore closure. The pkdcc1Mo phenotype was rescued by co-injection with 1ng of pkdcc1(mut) mRNA (C) and pkdcc2Mo phenotype was rescued by the co-injection with the 1ng of pkdcc2(mut) mRNA. (F-J) Four cell stage embryos were unilaterally injected with pkdcc1Mo (G), pkdcc2Mo (I) or coMo (F) and incubated until neural tube closure. Once again, the phenotype obtained by the absence of Pkdcc1 was rescued by the overexpression of pkdcc1(mut) mRNA (H) and pkdcc2Mo phenotype was rescued by co-injection of pkdcc2(mut) mRNA (J). n is the number of injected embryos and the percentage stands for the embryos with the observed defect. Image published in: Vitorino M et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Experiment + Assay Source Phenotypes and Disease Xla Wt + pkdcc.1 MO + NF12 (morphology) fig.S2.b Anatomical Phenotype abnormally delayed closure of blastopore Xla Wt + pkdcc.2 MO + NF16 (morphology) fig.S2.d Anatomical Phenotype abnormally delayed closure of blastopore Xla Wt + pkdcc.1 MO + NF16 (morphology) fig.S2.g Anatomical Phenotype abnormally delayed closure of neural tube Xla Wt + pkdcc.2 MO + NF16 (morphology) fig.S2.i Anatomical Phenotype abnormally delayed closure of neural tube Larger Image Printer Friendly View

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