Xenbase Image ID: 151057

Figure 1. Localization of Pk3 and Vangl2 in the gastrocoel roof plate (GRP).(A) Schematic neurula embryo section illustrating the position of the GRP (yellow). The dorsal (D)- ventral (V) axis is indicated. NP, neural plate; N, notochord; S, somites. (B) Enrichment of GFP-Pk3 at the base of cilium in GRP cells (arrowhead). Embryos were injected with GFP-Pk3 RNA (0.25 ng). Protein localization is detected by GFP fluorescence in stage 15 GRP explants. Cilium is stained by anti-acetylated α-tubulin (Ac-tub) antibody. (C) En face immunostaining of endogenous Vangl2 in the GRP of stage 15 embryos. The anterior (A) - posterior (P) axis is indicated. Note the accumulation of Vangl2 at anterior cell boundaries. There is no staining in the absence of primary antibody (data not shown). (D–D”) GRP cells coexpressing GFP-Pk3 and HA-Vangl2 (150 pg of RNA each) at stage 15. Arrowheads mark anterior cell borders. (E) Scheme of Pk3 constructs. (F) Physical interaction between Pk3 and Vangl2 in transfected HEK293T cells. The indicated FLAG-Pk3 proteins were pulled down using anti-FLAG-agarose beads, and co-precipitation of HA-Vangl2 was examined. Protein levels are shown by immunoblotting with anti-FLAG and anti-HA antibodies. Image published in: Chu CW et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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