Xenbase Image ID: 151059

Figure 3. Pk3 is required for the anterior polarization of Vangl2.(A,B) Immunostaining of Vangl2 (arrowheads) in stage 15 GRP cells from embryos injected with GFP RNA (0.2 ng, lineage tracer) and CoMO (15 ng, A,A’) or Pk3MO1 (15 ng, B,B’). (C,D) Embryos were injected with 2 ng of GFP-Pk3C RNA, and Vangl2 localization (C,C’) and cilia (marked by Ac-tub, D,D’) were visualized in GRP cells at stage 15 (C) and 17 (D) respectively. ZO-1 co-staining reveals cell boundaries. En face staining is shown, anterior is to the top. Representative images from three independent experiments are shown, with 6–10 explants per group. Image published in: Chu CW et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Experiment + Assay Source Phenotypes and Disease Xla Wt + prickle3 MO + NF15 (immunohistochemistry) fig.3.b, b^1 Anatomical Phenotype abnormal establishment of planar polarity Expression Phenotype mislocalised vangl2.L expression in gastrocoel roof plate Xla Wt + prickle3-GFP + NF15 (immunohistochemistry) fig.3.c, c^1 Anatomical Phenotype abnormal establishment of planar polarity Expression Phenotype mislocalised vangl2.L that expressed in plasma membrane Xla Wt + prickle3-GFP + NF15 (immunohistochemistry) fig.3.d, d^1 Anatomical Phenotype abnormally localised gastrocoel roof plate decreased length of archenteron decreased size of the gastrocoel roof plate Larger Image Printer Friendly View

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