Xenbase Image ID: 151069

Fig. S1. Cilia association of serotonin-containing vesicles and vesicle maturation. (A, B) Co-staining of serotonin (green) and cilia (red; anti acetylated-􀀀-tubulin) at stage 32 reveals occasional co-localization in the epidermis (white arrowheads). (B) Higher magnification of two MCCs in side view. Note the vesicular nature of serotonin staining in all cases. (C) Vesicle maturation in SSCs. Z-stack analysis of a single SSC stained for serotonin (red) and actin (phalloidin, green). Apical to basal horizontal optical sections (I-V) were used to reconstruct an orthogonal hypothetical model of vesicle maturation (lower panel in C). Vesicles localized basally were small (0.2 μm diameter), with a continuous increase in diameter to a maximum of 1 μm towards the apical pole of the cell. (D, D') Vesicular co-localization of serotonin (green) and peanut agglutinin (PNA; red) in the epidermis of Xenopus laevis tadpoles at stage 39. Scale bars in (D) and (D') represent 50 and 5μm, respectively. Image published in: Walentek P et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Throughout NF stage 32 epidermis epithelium cilium ciliated cell mucociliary epithelium Image source: Published Larger Image Printer Friendly View

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