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Xenbase Image ID: 151069

Fig. S1. Cilia association of serotonin-containing vesicles and vesicle maturation. (A, B) Co-staining of serotonin (green) and cilia (red; anti acetylated-􀀀-tubulin) at stage 32 reveals occasional co-localization in the epidermis (white arrowheads). (B) Higher magnification of two MCCs in side view. Note the vesicular nature of serotonin staining in all cases. (C) Vesicle maturation in SSCs. Z-stack analysis of a single SSC stained for serotonin (red) and actin (phalloidin, green). Apical to basal horizontal optical sections (I-V) were used to reconstruct an orthogonal hypothetical model of vesicle maturation (lower panel in C). Vesicles localized basally were small (0.2 μm diameter), with a continuous increase in diameter to a maximum of 1 μm towards the apical pole of the cell. (D, D') Vesicular co-localization of serotonin (green) and peanut agglutinin (PNA; red) in the epidermis of Xenopus laevis tadpoles at stage 39. Scale bars in (D) and (D') represent 50 and 5μm, respectively.

Image published in: Walentek P et al. (2014)

Copyright © 2014. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

tuba4b.LIMAGE:4683703tuba4, alpha tubulin, alpha-tubulinX.laevisThroughout NF stage 32epidermis
ciliated cell
mucociliary epithelium

Image source: Published

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