Xenbase Image ID: 151123

Figure 2. GR binding affinity to a complete HRE or a half HRE. (A) The DNA constructs used, the binding elements for GR and AR (BE1 and BE2) are highlighted in gray and the FoxA1 site indicated by a rectangle. These constructs are referred to in the text as HRE1/1 + Fox and HRE1/2 + Fox. (B) Nuclear GR concentration analyzed by [3H]-Dex quantification in nuclear extracts. (C) Autoradiogram showing the pattern of DMS methylation, empty circles signify partially protected bands, unprotected bands used as reference are marked with black dots. Columns in the diagrams to the left and right illustrate quantification of DMS methylation with error bars showing the average deviation of double samples. (D) GR-HRE binding based on DMS methylation protection plotted as a function of nuclear GR concentration. (E) Binding activity at the FoxA1 site based on DMS methylation protection, presumably by a DNA binding protein of endogenous origin. Image published in: Belikov S et al. (2016) © The Author(s) 2015. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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