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Figure 3. GR binding affinity to a complete HRE (HRE1/1 + Fox) and a half HRE (HRE1/2 + Fox) in presence of FoxA1 protein. Oocytes were injected with increasing amounts of GR mRNA, 3.5–8 ng, mixed with a constant amount of FoxA1 mRNA, 0.46 ng, resulting in 0.4 μM nuclear FoxA1 quantified as described (Supplementary Figure S3C). (A) Pattern of DMS methylation, as in Figure 2C. (B) GR-HRE binding based on DMS methylation protection of BE1 plotted as a function of nuclear GR concentration. (C) FoxA1-DNA binding based on DMS methylation protection plotted as a function of nuclear GR concentration. Image published in: Belikov S et al. (2016) © The Author(s) 2015. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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