XB-IMG-151190
Xenbase Image ID: 151190
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Figure 3. Mouse development with a mutated Ki-67 gene.(A) Table describing Ki-67 mutant mouse lines resulting from germline transmission of mutations generated by cytoplasmic injection of TALEN-encoding mRNA into zygotes. (B) Macroscopic appearance of littermate female mice at 10 weeks of age. Genotypes are specified. (C) IHC staining of Ki-67 in sagittal section of intestine from Mki67WT/WT, Mki67WT/2nt∆ and Mki672nt∆/2nt∆ mice. (D) Western blots of Ki-67 and cyclin A expression from intestine isolated from Mki67WT/WT, Mki67WT/2nt∆ and Mki672nt∆/2nt∆ mice. LC, loading control. (E) Western blot of Ki-67 in MEFs from WT, Mki67WT/2nt∆ and Mki672nt∆/2nt∆ mice. LC, loading control. (F) Flow cytometry profiles in WT, Mki67WT/2nt∆ and Mki672nt∆/2nt∆ MEFs showing EdU incorporation upon a 1 hr pulse and DNA content.DOI:
http://dx.doi.org/10.7554/eLife.13722.006Figure 3—figure supplement 1. Ki-67 mutant mice develop normally.(A) Pair of TALE-nucleases designed to target the initiator ATG of mouse Mki67 gene. (B) Sequencing traces of initiator ATG (underlined) of Mki67 gene in WT Mki67+/+(WT/WT), heterozygous Mki67+/2nt∆(WT/2nt∆) and homozygous Mki672nt∆/2nt∆(2nt∆/2nt∆) mice. (C) Sequencing traces of initiator ATG (underlined) of Mki67 gene in WT Mki67+/+(WT/WT), heterozygous Mki67+/21nt∆(WT/21nt∆) and homozygous Mki6721nt∆/21nt∆(21nt∆/21nt∆) mice. (D) WT Mki67+/+(+/+), heterozygous Mki67+/21nt∆(+/21nt∆) and homozygous Mki6721nt∆/21nt∆(21nt∆/21nt∆) mice.DOI:
http://dx.doi.org/10.7554/eLife.13722.007 Image published in: Sobecki M et al. (2016) © 2016, Sobecki et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |