XB-IMG-151200
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Figure 3—figure supplement 11. SILAC proteomics analysis of Ki-67 expression with a mutated ATG.(A) Scatterplot showing H/L m/z peak ratios (x-axis) against intensity levels (y-axis) from band 1 of SDS-PAGE purified chromatin, where H-labelled samples were Ki-67 mutant NIH-3T3 clone 14, L samples were W4 wild-type control. Most proteins have unaltered expression between the two cell lines (H/L ratio ≈ 1). Average H/L ratio of putative Ki-67 peptides (predicted by Maxquant on the basis of isotope profiles with the expected difference in m/z ratio from Ki-67 L-peptides positively identified by MS/MS) is shown in red. (B) Isotope profile of a light Ki-67 peptide from control cell chromatin identified by MS/MS, and the corresponding heavy peaks used for quantification. Data is provided in Figure 3—figure supplement 11—source data 1.DOI:
http://dx.doi.org/10.7554/eLife.13722.01710.7554/eLife.13722.018Figure 3—figure supplement 11—source data 1. SILAC quantitation of Ki-67 peptides from WT and Mki67-mutant NIH-3T3 cells.Table showing identity and H/L ratio of Ki-67 peptides purified from chromatin of NIH-3T3 Wt, Mki67 mutant clones 14 and 21, recovered in excised bands 1 (>250 kDa) or 2 (130-250 kDa). Data have been uploaded into the massIVE repository with accession number MSV000079492.DOI:
http://dx.doi.org/10.7554/eLife.13722.018 Image published in: Sobecki M et al. (2016) © 2016, Sobecki et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |