Xenbase Image ID: 151281

(A) Western blot of embryos injected with mRNA encoding triple FLAG-tagged β-catenin either alone or with increasing amounts of dkk. Numbers bellow FLAG blot represent relative abundance of FLAG epitope compared to abundance in embryos injected with 500pg triple FLAG-tagged β-catenin. Numbers are normalized to the amount of actin loading control detected in the same blot. (B) Number of peaks called by MACS and Homer peak callers. Final peak set was selected by taking the intersection from both peak callers for each sample and then the intersection of all three samples. (C) Chromatin Immunoprecipitaion (ChIP) followed by qPCR using uninjected control (UC) embryos or embryos injected with 500pg triple FLAG-tagged β-catenin either alone or with dkk. Primers were designed to amplify the central region of selected peaks. The negative region is 10 kb from peak_16965. MACS peak score is given for each peak. We note the progressive decline in binding at negative control regions progressively further from the peak, expected from incomplete sonication of chromatin. Values are normalized to input and fold change is taken compared to uninjected control (UC). Error bars are standard deviation. Image published in: Kjolby RAS and Harland RM (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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