XB-IMG-151287
Xenbase Image ID: 151287
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Fig. 2. Integrating exogenous DNA into Xenopus using genetic editing tools. Outline of the various knock-in strategies that have been employed to insert DNA into a targeted genomic locus in Xenopus. (A) Nakade et al. described the use of TALENs and microhomology-mediated end joining (MMEJ, TAL-PITCh) to integrate a fluorescent protein (eg. GFP) at the end of the coding region 5′ to the endogenous stop codon. (B) Shi et al. utilized CRISPR-Cas editing to insert plasmid DNA harboring a known pancreas tissue enhancer element (Elastase promoter) driving GFP, into the intron of their target gene. (C) Jaffe et al., used targeting constructs containing allele-specific homology arms to insert fluorescent proteins into a sgRNA-targeted exon, thereby visualizing cells in which specific gene function was abrogated. TAA; stop codon, FokI; Fok1 nuclease, GFP; green fluorescent protein, pA; poly-A tail, sgRNA; guide RNA for CRISPR. Image published in: Tandon P et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |