XB-IMG-151523
Xenbase Image ID: 151523
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Fig. 6. Compromised mitochondrial homeostasis in axons deficient for Tctp. (A) Relative ATP levels per retina normalised to total protein. Mean±s.e.m.; n=17 per condition, ***P<0.0001, unpaired t-test. (B) Representative RGC growth cones loaded with TMRM from con-MO-injected and tctp-MO-injected embryos. (C) Quantification of TMRM fluorescence intensity in the mitochondria-rich growth cone (GC) central domain. Mean±s.e.m.; ***P=0.0002, Mann–Whitney test. (D) Quantification of TMRM fluorescence intensity of individual mitochondria along the axonal compartment. Mean±s.e.m.; **P<0.0012, Mann–Whitney test. Up to ten replicate experiments were performed per condition, totalling 427 growth cones and 4918 single mitochondria analysed. (E) Schematic of the approach used to examine mitochondrial density in RGC axons in vivo. (F) Micrographs of RGC axons co-labelled with mt-GFP and gap-RFP, plus quantification of axonal mitochondrial density. n, number of axons analysed; ***P=0.0002, unpaired t-test. In box plots, whiskers cover 5th-95th percentile and ‘+’ indicates the mean. Boxed areas in images are enlarged to the right. Scale bars: 5 μm. Image published in: Roque CG et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |