XB-IMG-151524
Xenbase Image ID: 151524
|
Fig. 7. Altered mitochondrial dynamics in Tctp-depleted axons. (A) Ratio of mitochondrial to nuclear DNA determined by qPCR in control and Tctp-depleted retinas. Mean±95% confidence interval; n=7 paired retinas per condition; P=0.23, Mann–Whitney test. (B) Tctp morphants show unaltered Pgc1α expression levels in the CNS as evaluated by western blot analysis of stage 37/38 embryos using an anti-Pgc1α antibody. n=3 independent samples; P=0.5955, unpaired t-test. (C) Tctp morphants show unaltered expression of mitochondria-related genes as assessed by RT-qPCR using eye RNA extracts. Mean±95% confidence interval; n=9 retinas per condition, Mann–Whitney test. (D) Tctp morphants show unaltered cytochrome c expression levels in the CNS, as evaluated by western blot analysis of stage 37/38 embryos using an anti-cytochrome c antibody. n=3 independent samples; P=0.5989, unpaired t-test. (E) Control and Tctp-depleted RGCs have similar levels of cox5a expression. Mean±s.e.m.; n=∼20. GCL, P=0.2026; IPL, P=0.2668; Mann–Whitney test. (F) Representative kymographs (time-space plots) of MitoTracker-labelled RGC axonal mitochondria in control and Tctp morphant backgrounds. The vertical and horizontal axes represent time and spatial position, respectively (e.g. a vertical line indicates a stationary mitochondrion). (G) Summary of changes in axonal mitochondrial dynamics (statistical significance determined using Fisher's exact test). (H) Relative mitochondrial motility and mean net movement in control and Tctp-depleted RGC axons. Box plot whiskers indicate 5th-95th percentile. Right: Mean±s.e.m.; *P<0.0117, Mann–Whitney test. (I) Analysis of fast mitochondrial transport. Mean±s.e.m.; anterograde direction, P=0.9468; retrograde direction, P=0.7308; Mann–Whitney test. (J) Average duration of mitochondrial pauses in control and Tctp-depleted RGC axons. Box plot whiskers indicate 5-95 percentile and ‘+’ the mean; P=0.902, Mann–Whitney test. Permanently stationary mitochondria were excluded from this analysis. (K) Average number and frequency distributions of mitochondrial pauses. Mean±s.e.m.; P=0.317, Mann–Whitney test. Scale bars: 50 μm in E; 5 μm in F. n.s., not significant. Image published in: Roque CG et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |